VCPIP1 Double Nickase Plasmid (h): sc-409332-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VCPIP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VCPIP1 Double Nickase Plasmid (h) and VCPIP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VCPIP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VCPIP1 Antibody (C-12): sc-515291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VCPIP1 Double Nickase Plasmid (h) | sc-409332-NIC | 20 µg | $410.00 |
VCPIP1 (valosin-containing protein interacting protein 1) encodes a deubiquitinating enzyme that associates with the AAA+ ATPase VCP/p97 to regulate ubiquitin-dependent protein quality control. Through its OTU-domain catalytic activity, VCPIP1 participates in endoplasmic reticulum–associated degradation (ERAD), membrane trafficking, and proteostasis by editing ubiquitin chains on select substrates and coordinating their processing by the VCP/p97 machinery. Disruption of VCP/p97-linked ubiquitin homeostasis is connected to cellular stress signaling, impaired organelle dynamics, and neurodegeneration-associated pathways, making VCPIP1 a relevant node for studying how ubiquitin remodeling influences stress resilience and degradation networks. Altered regulation of these pathways is also studied in the context of cancer cell adaptation to proteotoxic stress and dysregulated ubiquitin signaling.
VCPIP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VCPIP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VCPIP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VCPIP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VCPIP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.