UXS1 Double Nickase Plasmid (h): sc-413346-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UXS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UXS1 Double Nickase Plasmid (h) and UXS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UXS1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UXS1 Double Nickase Plasmid (h) | sc-413346-NIC | 20 µg | $410.00 | |||
UXS1 Double Nickase Plasmid (h2) | sc-413346-NIC-2 | 20 µg | $410.00 |
Human UXS1 encodes UDP-glucuronic acid decarboxylase 1, a cytosolic enzyme that converts UDP-glucuronic acid to UDP-xylose, an essential sugar donor for proteoglycan and glycosaminoglycan biosynthesis. By supplying UDP-xylose, UXS1 supports initiation and elongation of extracellular matrix glycoconjugates, influencing cell–matrix interactions, secretory pathway glycosylation outputs, and tissue homeostasis. Perturbation of this nucleotide-sugar pathway can alter proteoglycan composition and downstream signaling environments that shape cellular adhesion, migration, and differentiation programs. UXS1 dysregulation is therefore relevant to mechanistic studies of connective tissue biology and disorders linked to abnormal proteoglycan assembly.
UXS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UXS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UXS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UXS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UXS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.