Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USP11 CRISPR/Cas9 KO Plasmid (h): sc-403368
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP11 Antibody (C-6): sc-365528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP11 CRISPR/Cas9 KO Plasmid (h) | sc-403368 | 20 µg | $397.00 |
Overview
USP11 is a human ubiquitin-specific protease that removes ubiquitin from protein substrates to regulate their stability, localization, and signaling output. Through deubiquitination, USP11 contributes to proteostasis control and modulates pathways linked to DNA damage responses, transcriptional regulation, and cell-cycle progression. Reported interactions place USP11 within ubiquitin-dependent regulatory networks that influence stress signaling and chromatin-associated processes. Dysregulated USP11 activity has been associated with altered cellular growth control and genome maintenance phenotypes, supporting its relevance in cancer biology and other disorders involving ubiquitin signaling imbalance.
USP11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the USP11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the USP11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the USP11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP11 protein expression.
This CRISPR knockout system enables efficient generation of USP11-deficient cell models for investigation of USP11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting USP11 exon(s) critical for USP11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple USP11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP11 CRISPR/Cas9 KO Plasmid (h) and USP11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the USP11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP11 HDR Plasmid (h) and USP11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by USP11 homology arms to support homology-directed repair at defined USP11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.