UNCX CRISPR/Cas9 KO Plasmid (m): sc-423613
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UNCX CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UNCX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UNCX CRISPR/Cas9 KO Plasmid (m) | sc-423613 | 20 µg | $397.00 |
Overview
Uncx encodes the homeobox transcription factor UNCX, a nuclear DNA-binding regulator implicated in embryonic patterning and cell fate specification in the mouse. UNCX activity contributes to developmental gene regulatory networks that coordinate neuronal differentiation and somite-derived tissue organization, interfacing with transcriptional programs controlling regional identity and maturation. Altered Uncx function has been associated with defects in axial/somitic development and neurodevelopmental processes, making it a useful node for studying congenital phenotypes and lineage commitment. In experimental systems, UNCX serves as a marker and modulator of differentiation states where precise transcriptional control is required.
UNCX CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Uncx gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Uncx together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Uncx open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UNCX protein expression.
This CRISPR knockout system enables efficient generation of Uncx-deficient cell models for investigation of UNCX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Uncx exon(s) critical for UNCX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Uncx genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UNCX CRISPR/Cas9 KO Plasmid (m) and UNCX CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Uncx locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UNCX HDR Plasmid (m) and UNCX HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Uncx homology arms to support homology-directed repair at defined Uncx target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.