TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (h): sc-407927

Overview

TTF1 (transcription termination factor 1; also known as TTFC) is a nucleolar DNA-binding protein that regulates RNA polymerase I transcription termination and contributes to rDNA chromatin organization and nucleolus architecture. By binding specific terminator elements within ribosomal DNA repeats, TTF1 helps coordinate rRNA synthesis with replication and repair events at the rDNA locus, influencing ribosome biogenesis and proteostasis. Altered control of Pol I transcription and nucleolar function is a hallmark of proliferative stress and is frequently studied in the context of oncogenic signaling, genome instability, and cellular senescence. As a result, TTF1 is commonly interrogated in pathways linking nucleolar homeostasis, transcriptional regulation, and cell-cycle checkpoint responses.

TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TTF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TTF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TTF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TTF/Transcription Termination Factor/TTF1 protein expression.

This CRISPR knockout system enables efficient generation of TTF1-deficient cell models for investigation of TTF/Transcription Termination Factor/TTF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting TTF1 exon(s) critical for TTF/Transcription Termination Factor/TTF1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple TTF1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (h) and TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TTF1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by TTF/Transcription Termination Factor/TTF1 HDR Plasmid (h) and TTF/Transcription Termination Factor/TTF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TTF1 homology arms to support homology-directed repair at defined TTF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.