TRPV5 Antibody (B-8): sc-398345
- TRPV5 Antibody (B-8) is a mouse monoclonal IgG2a κ TRPV5 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 626-723 mapping at the C-terminus of TRPV5 of rat origin
- TRPV5 Antibody (B-8) is recommended for detection of TRPV5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TRPV5 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for TRPV5 Antibody (B-8) for WB applications. This reagent is now offered in a bundle with TRPV5 Antibody (B-8) (see ordering information below).
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TRPV5 Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TRPV5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRPV5 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRPV5 protein, consisting of 729 amino acids, plays a crucial role in calcium homeostasis by regulating the rate-limiting step of vitamin D3-mediated calcium reabsorption in the kidney and intestine. TRPV5 structure features six transmembrane domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and three ankyrin repeat regions, which are essential for function and interaction with other proteins. Notably, the 5′-flanking region of TRPV5 contains four putative vitamin D3-responsive elements, highlighting regulatory relationship with vitamin D3 levels. TRPV5 is predominantly expressed in the kidney, jejunum, and pancreas, with lower expression levels found in the testis, prostate, placenta, brain, colon, and rectum. Understanding TRPV5 function and structure is vital, as TRPV5 not only contributes to calcium reabsorption but also plays a significant role in maintaining overall mineral balance in the body, which is essential for various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRPV5 Antibody (B-8) References:
- Molecular determinants in TRPV5 channel assembly. | Chang, Q., et al. 2004. J Biol Chem. 279: 54304-11. PMID: 15489237
- Regulation of the epithelial calcium channel TRPV5 by extracellular factors. | Topala, CN., et al. 2007. Curr Opin Nephrol Hypertens. 16: 319-24. PMID: 17565273
- TRPV5 and TRPV6 in transcellular Ca(2+) transport: regulation, gene duplication, and polymorphisms in African populations. | Peng, JB. 2011. Adv Exp Med Biol. 704: 239-75. PMID: 21290300
- TRPV5 in renal tubular calcium handling and its potential relevance for nephrolithiasis. | van der Wijst, J., et al. 2019. Kidney Int. 96: 1283-1291. PMID: 31471161
- Structure-based characterization of novel TRPV5 inhibitors. | Hughes, TE., et al. 2019. Elife. 8: PMID: 31647410
- Structural basis of TRPV5 regulation by physiological and pathophysiological modulators. | Fluck, EC., et al. 2022. Cell Rep. 39: 110737. PMID: 35476976
- Alterations in the microenvironment and the effects produced of TRPV5 in osteoporosis. | Luo, ZH., et al. 2023. J Transl Med. 21: 327. PMID: 37198647
- Probing Conformational Transition of TRPV5 Induced by Mechanical Force Using Coarse-Grained Molecular Dynamics. | Gu, Y., et al. 2023. J Chem Inf Model. 63: 6768-6777. PMID: 37871325
- The ion channel TRPV5 regulates B-cell signaling and activation. | Mahtani, T., et al. 2024. Front Immunol. 15: 1386719. PMID: 38694510
- Calmodulin regulates TRPV5 intracellular trafficking and plasma membrane abundance. | Zuidscherwoude, M., et al. 2024. J Physiol. 602: 6871-6888. PMID: 39576090
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRPV5 Antibody (B-8) | sc-398345 | 200 µg/ml | $322.00 | |||
TRPV5 Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-552120 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRPV5 Antibody (B-8) AC | sc-398345 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRPV5 Antibody (B-8) HRP | sc-398345 HRP | 200 µg/ml | $322.00 | |||
TRPV5 Antibody (B-8) FITC | sc-398345 FITC | 200 µg/ml | $336.00 | |||
TRPV5 Antibody (B-8) PE | sc-398345 PE | 200 µg/ml | $349.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 488 | sc-398345 AF488 | 200 µg/ml | $364.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 546 | sc-398345 AF546 | 200 µg/ml | $364.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 594 | sc-398345 AF594 | 200 µg/ml | $364.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 647 | sc-398345 AF647 | 200 µg/ml | $364.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 680 | sc-398345 AF680 | 200 µg/ml | $364.00 | |||
TRPV5 Antibody (B-8) Alexa Fluor® 790 | sc-398345 AF790 | 200 µg/ml | $364.00 |