TRAP100 CRISPR/Cas9 KO Plasmid (h): sc-417318
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRAP100 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRAP100 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAP100 CRISPR/Cas9 KO Plasmid (h) | sc-417318 | 20 µg | $397.00 |
Overview
MED24 encodes TRAP100, an essential subunit of the Mediator complex that bridges sequence-specific transcription factors with RNA polymerase II to coordinate promoter-enhancer communication and transcription initiation. TRAP100 contributes to signal-dependent gene regulation programs, including nuclear receptor–driven transcription and broader chromatin-associated control of cell cycle progression, differentiation, and stress responses. Disruption of Mediator subunits, including MED24, can perturb transcriptional networks linked to developmental abnormalities and oncogenic transcriptional rewiring, making MED24 a useful node for studying pathway-level control of gene expression. MED24/TRAP100 function is therefore relevant to investigations of transcriptional dysregulation in cancer biology and other diseases driven by altered gene regulatory circuitry.
TRAP100 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MED24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MED24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MED24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRAP100 protein expression.
This CRISPR knockout system enables efficient generation of MED24-deficient cell models for investigation of TRAP100 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MED24 exon(s) critical for TRAP100 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MED24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRAP100 CRISPR/Cas9 KO Plasmid (h) and TRAP100 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MED24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRAP100 HDR Plasmid (h) and TRAP100 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MED24 homology arms to support homology-directed repair at defined MED24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.