Anticorps TMPRSS2 (H-4) recommandé pour la détection de TMPRSS2 catalytic chain d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps TMPRSS2 (H-4): sc-515727

Anticorps TMPRSS2 (H-4) est évalué 4.0 de 5 de 7.
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Fiches techniques

    • Anti-L'anticorps TMPRSS2 H-4 est un monoclonal IgG1 κ L'Anticorps TMPRSS2, cité dans 51 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 296-345 situés dans une région interne de TMPRSS2 de human
    • Anti-L'Anticorps TMPRSS2 (H-4) est recommandé pour la détection de TMPRSS2 catalytic chain d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps TMPRSS2 (H-4) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de TMPRSS2 (H-4): sc-515727.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps TMPRSS2 (H-4) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps TMPRSS2 (H-4) (voir Informations pour la commande ci-dessous).
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Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Posée par: CGJvirus
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Répondue par: Technical Support
Date de publication: 2021-07-22

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Posée par: ealeksa2
Thank you for your question. TMPRSS2 Antibody (H-4): sc-515727 was raised against amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin, which correspond to the extracellular domain of the protein. The antibody has not been validated for flow cytometry.
Répondue par: Technical Support Europe
Date de publication: 2020-10-08

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

Posée par: LiMS
Thank you for your question. We recommend using 1 µg antibody (5 µl) per 1 million cells.
Répondue par: Tech Support Europe
Date de publication: 2020-08-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Posée par: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Technical Support
Date de publication: 2017-02-27
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Rated 4 de 5 de par Good antibody I used this antibody fo immunofluorescence in Calu-3 and Hek293 cells. I actually visualised the antibody only in Calu-3 cells, as expected. Good staining.
Date de publication: 2021-05-04
Rated 5 de 5 de par Works for immunofluorescence Used it to visualize ectopically expressed TMPRSS2 in HEK293 cells by IF. Gave good signal with apparent cell surface expression, as expected. This was a courtesy replacement for sc-101847, which gave no signal at all.
Date de publication: 2020-12-30
Rated 4 de 5 de par Working in WB human TMPRSS2 expressed in mice was successfully detected by WB.
Date de publication: 2020-11-21
Rated 4 de 5 de par Good product and works well. I bought a month ago and it works for detecting TMPRSS2 proteins.
Date de publication: 2020-07-14
Rated 5 de 5 de par Excellent in western blotting Dilution 1.1000 Western Blotting cell lysate of MDA, C2C12, HepG2 Very stong and specific signals. Detection changes according the cell type (different glycosilate forms)m
Date de publication: 2020-05-18
Rated 1 de 5 de par Kein spezifisches Signal Bei der Expression in Oozyten haben wir kein spezifisches Signal in Ganzzelllysaten detektiert. Starkes Signal bei etwa 55 kDa ist unspezifisch und auch in Kontrollgruppe vorhanden.
Date de publication: 2020-05-18
Rated 5 de 5 de par effective i recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:100 dilution), band is clear
Date de publication: 2017-08-15
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