Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TMEM38A Double Nickase Plasmid (m): sc-428763-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM38A Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TMEM38A Double Nickase Plasmid (m) and TMEM38A Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tmem38a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TMEM38A Antibody (G-12): sc-390054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM38A Double Nickase Plasmid (m) | sc-428763-NIC | 20 µg | $410.00 | |||
TMEM38A Double Nickase Plasmid (m2) | sc-428763-NIC-2 | 20 µg | $410.00 |
Tmem38a encodes TMEM38A, an endoplasmic reticulum membrane protein implicated in intracellular ion homeostasis and excitation–contraction coupling through regulation of calcium release dynamics. In mouse, TMEM38A is linked to skeletal muscle physiology and contributes to processes that shape ER calcium handling, membrane excitability, and downstream signaling pathways responsive to calcium flux. Perturbation of TMEM38A activity can alter calcium-dependent transcriptional programs and stress responses that influence myofiber function and metabolic adaptation. These features make TMEM38A a relevant target for studying mechanisms underlying muscle performance, myopathy-associated phenotypes, and broader disorders connected to disrupted calcium signaling.
TMEM38A Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tmem38a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tmem38a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tmem38a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tmem38a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.