TMEM192 Double Nickase Plasmid (h): sc-415021-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM192 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TMEM192 Double Nickase Plasmid (h) and TMEM192 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TMEM192. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TMEM192 Antibody (F-12): sc-518207
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM192 Double Nickase Plasmid (h) | sc-415021-NIC | 20 µg | $410.00 | |||
TMEM192 Double Nickase Plasmid (h2) | sc-415021-NIC-2 | 20 µg | $410.00 |
TMEM192 encodes a multi-pass transmembrane protein enriched on lysosomal membranes and implicated in maintaining lysosome identity, membrane organization, and proteolytic homeostasis. By influencing lysosomal trafficking and turnover, TMEM192 intersects with pathways controlling endolysosomal maturation, autophagy–lysosome function, and cellular stress responses. Dysregulated lysosomal dynamics are broadly relevant to neurodegeneration, infection biology, and cancer cell metabolism, making TMEM192 a useful target for probing how lysosome-dependent processes shape cell physiology. In human cell models, TMEM192 perturbation can help clarify links between lysosomal membrane composition and downstream catabolic signaling networks.
TMEM192 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TMEM192 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TMEM192. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TMEM192 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TMEM192-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.