Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TEL Double Nickase Plasmid (h): sc-402207-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TEL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TEL Double Nickase Plasmid (h) and TEL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ETV6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TEL Antibody (E-1): sc-166835
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TEL Double Nickase Plasmid (h) | sc-402207-NIC | 20 µg | $410.00 | |||
TEL Double Nickase Plasmid (h2) | sc-402207-NIC-2 | 20 µg | $410.00 |
ETV6 (TEL) encodes an ETS-family transcription factor that functions predominantly as a transcriptional repressor controlling hematopoietic stem and progenitor cell maintenance, lineage commitment, and homeostatic proliferation. TEL integrates signaling cues with chromatin regulation through interactions with corepressors and histone deacetylase–associated complexes, shaping gene expression programs involved in differentiation and apoptosis. Disruption of ETV6 contributes to genomic instability and altered transcriptional control, and ETV6 is recurrently implicated in hematologic malignancies through translocations, point mutations, and haploinsufficiency. As a central node in blood development and leukemogenic gene fusions, TEL is widely studied for its role in oncogenic transcriptional networks and aberrant kinase signaling pathways.
TEL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ETV6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ETV6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ETV6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ETV6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.