Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TARDBP Double Nickase Plasmid (h): sc-401890-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TARDBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TARDBP Double Nickase Plasmid (h) and TARDBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TARDBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TARDBP Antibody (E-10): sc-376311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TARDBP Double Nickase Plasmid (h) | sc-401890-NIC | 20 µg | $410.00 | |||
TARDBP Double Nickase Plasmid (h2) | sc-401890-NIC-2 | 20 µg | $410.00 |
TARDBP (TDP-43) is a ubiquitously expressed RNA/DNA-binding protein that regulates multiple layers of RNA metabolism, including pre-mRNA splicing, transcript stability, and transport, and it also participates in transcriptional regulation. It localizes predominantly to the nucleus but can shuttle to the cytoplasm and is recruited to stress granules during cellular stress, linking it to proteostasis and RNA quality-control processes. TARDBP-dependent RNA processing helps maintain neuronal homeostasis by shaping splicing programs and preventing aberrant cryptic exon inclusion across long neuronal transcripts. Dysregulation, mislocalization, and aggregation of TDP-43 are strongly associated with neurodegenerative disease mechanisms, including ALS and frontotemporal lobar degeneration, and are also studied in broader contexts of RNA-binding protein dysfunction.
TARDBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TARDBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TARDBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TARDBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TARDBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.