Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tankyrase-2 CRISPR/Cas9 KO Plasmid (h): sc-403925
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tankyrase-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tankyrase-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tankyrase-2 CRISPR/Cas9 KO Plasmid (h) | sc-403925 | 20 µg | $397.00 |
Overview
TNKS2 encodes tankyrase-2, a PARP family poly(ADP-ribose) polymerase that regulates protein stability and signaling through PARylation-dependent turnover. Tankyrase-2 promotes ubiquitin-mediated degradation of substrates such as AXIN, thereby modulating Wnt/β-catenin pathway activity and downstream transcriptional programs controlling proliferation and differentiation. It also contributes to telomere maintenance via interaction with TRF1 and influences mitotic progression and spindle organization. Dysregulated tankyrase activity has been linked to aberrant Wnt signaling and genome maintenance defects relevant to oncogenic transformation and other proliferative pathologies.
Tankyrase-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNKS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNKS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNKS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tankyrase-2 protein expression.
This CRISPR knockout system enables efficient generation of TNKS2-deficient cell models for investigation of Tankyrase-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNKS2 exon(s) critical for Tankyrase-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNKS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tankyrase-2 CRISPR/Cas9 KO Plasmid (h) and Tankyrase-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNKS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tankyrase-2 HDR Plasmid (h) and Tankyrase-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNKS2 homology arms to support homology-directed repair at defined TNKS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.