Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAL1 CRISPR/Cas9 KO Plasmid (h2): sc-401101-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAL1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAL1 Antibody (C-4): sc-393287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAL1 CRISPR/Cas9 KO Plasmid (h2) | sc-401101-KO-2 | 20 µg | $397.00 |
Overview
TAL1 (T-cell acute lymphocytic leukemia protein 1) is a basic helix–loop–helix transcription factor that regulates lineage specification and differentiation programs in hematopoietic development, particularly erythroid and T-cell compartments. It functions within multiprotein transcriptional complexes with partners such as E2A/TCF3, LMO2, LDB1, and GATA factors to control enhancer activity and chromatin accessibility at genes governing proliferation and maturation. TAL1 integrates signaling and transcriptional networks that shape hematopoietic stem/progenitor cell fate decisions and maintains context-dependent transcriptional states. Dysregulated TAL1 expression or enhancer hijacking is strongly associated with T-cell acute lymphoblastic leukemia biology and broader perturbations of hematopoietic transcriptional circuitry.
TAL1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the TAL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TAL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TAL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAL1 protein expression.
This CRISPR knockout system enables efficient generation of TAL1-deficient cell models for investigation of TAL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TAL1 exon(s) critical for TAL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TAL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAL1 CRISPR/Cas9 KO Plasmid (h) and TAL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TAL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAL1 HDR Plasmid (h) and TAL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TAL1 homology arms to support homology-directed repair at defined TAL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.