T2R14 Double Nickase Plasmid (h): sc-410582-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T2R14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- T2R14 Double Nickase Plasmid (h) and T2R14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TAS2R14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T2R14 Double Nickase Plasmid (h) | sc-410582-NIC | 20 µg | $410.00 |
TAS2R14 encodes the human bitter taste receptor T2R14, a class A GPCR that detects diverse bitter compounds and couples to heterotrimeric G proteins to regulate second-messenger signaling. Receptor activation typically engages PLCβ-dependent pathways that elevate intracellular calcium and modulate MAPK and other downstream signaling networks, influencing secretion, motility, and innate defense programs in extraoral tissues. TAS2R14 expression has been reported beyond the gustatory system, including in airway and gastrointestinal epithelia, where chemosensory signaling can shape barrier and inflammatory responses. Altered bitter receptor signaling has been investigated in the context of airway hyperreactivity, chronic inflammatory conditions, and tumor-associated signaling changes, supporting its relevance in disease-mechanism studies.
T2R14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TAS2R14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAS2R14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAS2R14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAS2R14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.