Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SYP/Synaptophysin CRISPR/Cas9 KO Plasmid (m): sc-423242
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SYP/Synaptophysin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SYP/Synaptophysin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SYP/Synaptophysin Antibody (D-4): sc-17750
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SYP/Synaptophysin CRISPR/Cas9 KO Plasmid (m) | sc-423242 | 20 µg | $397.00 |
Overview
Syp encodes synaptophysin (SYP), an abundant synaptic vesicle membrane glycoprotein widely used as a presynaptic marker in the mouse nervous system. SYP participates in synaptic vesicle trafficking, exo-endocytosis, and activity-dependent recycling through interactions with vesicle fusion and endocytic machinery, helping maintain neurotransmitter release competence. Altered synaptophysin expression and presynaptic vesicle dynamics have been linked to disrupted synaptic connectivity and are frequently evaluated in studies of neurodevelopmental and neurodegenerative mechanisms. As a core component of presynaptic terminals, SYP provides a functional entry point for dissecting circuit-level changes that emerge from impaired vesicle cycling.
SYP/Synaptophysin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SYP/Synaptophysin protein expression.
This CRISPR knockout system enables efficient generation of Syp-deficient cell models for investigation of SYP/Synaptophysin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syp exon(s) critical for SYP/Synaptophysin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SYP/Synaptophysin CRISPR/Cas9 KO Plasmid (m) and SYP/Synaptophysin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SYP/Synaptophysin HDR Plasmid (m) and SYP/Synaptophysin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syp homology arms to support homology-directed repair at defined Syp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.