Syntaxin 1 Antibody (SP7): sc-65510

Syntaxin 1 Antibody (SP7) is a mouse monoclonal IgG2a antibody that detects Syntaxin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Syntaxin 1 plays a crucial role in vesicular transport, vital for proper functioning of eukaryotic cells. Syntaxin 1 belongs to the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) family, essential for synaptic vesicle fusion with plasma membrane, facilitating neurotransmitter release. Syntaxin 1 primarily localizes in presynaptic terminals of neurons, interacting with other SNARE proteins, including VAMP and SNAP-25, to form a stable SNARE complex. This complex enables vesicle docking and fusion, ensuring regulated neurotransmitter release fundamental for neuronal communication. Proper functioning of Syntaxin 1 and associated protein interactions are essential for synaptic transmission, where dysregulation can lead to neurological disorders. Anti-Syntaxin 1 antibody (SP7) serves as an invaluable tool for researchers studying synaptic mechanisms and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Syntaxin 1 Antibody (SP7) References:

1. Decreased expression of t-SNARE, syntaxin 1, and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets: restoration of decreased t-SNARE proteins improves impaired insulin secretion.  |  Nagamatsu, S., et al. 1999. Diabetes. 48: 2367-73. PMID: 10580425
2. D53 is a novel endosomal SNARE-binding protein that enhances interaction of syntaxin 1 with the synaptobrevin 2 complex in vitro.  |  Proux-Gillardeaux, V., et al. 2003. Biochem J. 370: 213-21. PMID: 12376003
3. The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.  |  Hatsuzawa, K., et al. 2003. J Biol Chem. 278: 31159-66. PMID: 12782620
4. Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1.  |  Margittai, M., et al. 2003. Proc Natl Acad Sci U S A. 100: 15516-21. PMID: 14668446
5. Phosphorylation of syntaphilin by cAMP-dependent protein kinase modulates its interaction with syntaxin-1 and annuls its inhibitory effect on vesicle exocytosis.  |  Boczan, J., et al. 2004. J Biol Chem. 279: 18911-9. PMID: 14985338
6. Functional analysis of phosphorylation on Saccharomyces cerevisiae syntaxin 1 homologues Sso1p and Sso2p.  |  Yuan, Q. and Jäntti, J. 2010. PLoS One. 5: e13323. PMID: 20948969
7. Nesca, a novel neuronal adapter protein, links the molecular motor kinesin with the pre-synaptic membrane protein, syntaxin-1, in hippocampal neurons.  |  MacDonald, JI., et al. 2012. J Neurochem. 121: 861-80. PMID: 22404429
8. Extracellular CADM1 interactions influence insulin secretion by rat and human islet β-cells and promote clustering of syntaxin-1.  |  Zhang, C., et al. 2016. Am J Physiol Endocrinol Metab. 310: E874-85. PMID: 27072493
9. Axonal transport and targeting of the t-SNAREs SNAP-25 and syntaxin 1 in the peripheral nervous system.  |  Li, JY., et al. 1996. Eur J Cell Biol. 70: 12-22. PMID: 8738415
10. Protein-protein interactions of the yeast Golgi t-SNARE Sed5 protein distinct from its neural plasma membrane cognate syntaxin 1.  |  Kosodo, Y., et al. 1998. Biochem Biophys Res Commun. 250: 212-6. PMID: 9753609

The preferred Syntaxin 1 antibody is Syntaxin 1 Antibody (HPC-1): sc-12736.