Synaptotagmin III CRISPR/Cas9 KO Plasmid (m): sc-423245
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptotagmin III CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptotagmin III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptotagmin III CRISPR/Cas9 KO Plasmid (m) | sc-423245 | 20 µg | $397.00 |
Overview
Mouse Syt3 encodes Synaptotagmin III, a membrane-anchored C2 domain protein that functions as a Ca2+ sensor coupling calcium influx to regulated vesicle trafficking and membrane fusion. Synaptotagmin III contributes to synaptic vesicle exocytosis and endocytic recycling, helping shape neurotransmitter release kinetics and short-term synaptic plasticity. Through Ca2+-dependent interactions with phospholipids and SNARE-associated machinery, it influences activity-dependent trafficking pathways in neurons and other secretory cell types. Altered synaptotagmin family signaling has been linked to mechanisms relevant to neurodevelopmental and neurodegenerative phenotypes, supporting Syt3 as a target for interrogating synaptic dysfunction.
Synaptotagmin III CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syt3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syt3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syt3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptotagmin III protein expression.
This CRISPR knockout system enables efficient generation of Syt3-deficient cell models for investigation of Synaptotagmin III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syt3 exon(s) critical for Synaptotagmin III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syt3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptotagmin III CRISPR/Cas9 KO Plasmid (m) and Synaptotagmin III CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syt3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptotagmin III HDR Plasmid (m) and Synaptotagmin III HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syt3 homology arms to support homology-directed repair at defined Syt3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.