SSB-1 Double Nickase Plasmid (h): sc-408087-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSB-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SSB-1 Double Nickase Plasmid (h) and SSB-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPSB1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSB-1 Double Nickase Plasmid (h) | sc-408087-NIC | 20 µg | $410.00 | |||
SSB-1 Double Nickase Plasmid (h2) | sc-408087-NIC-2 | 20 µg | $410.00 |
Human SPSB1 encodes SSB-1, a SPRY domain–containing adaptor protein that links specific substrates to Cullin-RING ubiquitin ligase complexes via its SOCS box, promoting ubiquitination and proteasomal turnover. Through this E3 ligase–associated activity, SSB-1 contributes to regulation of protein stability in pathways governing cytokine and innate immune signaling, including modulation of JAK/STAT-related responses. SPSB1 has been studied as a negative regulator of inflammatory signaling and interferon-stimulated gene programs, positioning it as a useful node for dissecting feedback control in host defense. Altered SPSB1 expression or function may influence immune dysregulation and inflammation-associated phenotypes, making it relevant for mechanistic studies in immune and epithelial cell models.
SSB-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPSB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPSB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPSB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPSB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.