Superoxide Dismutase 2/SOD2 Antibody (E-7): sc-515068

SOD-2 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 137-162 within an internal region of Superoxide Dismutase 2 (SOD2) of human origin, and is reactive with human species. SOD2, also known as manganese superoxide dismutase (MnSOD), is a critical mitochondrial enzyme responsible for converting superoxide anions, a type of reactive oxygen species (ROS), into hydrogen peroxide and oxygen. This enzymatic activity is vital for cellular homeostasis, as superoxide anions are byproducts of mitochondrial respiration that, if not neutralized, can lead to oxidative damage of proteins, lipids, and DNA. SOD2′s function is crucial in preventing oxidative stress, which has been implicated in the pathogenesis of various conditions, including neurodegenerative diseases like Alzheimer′s and Parkinson′s, ischemic heart disease, and cancer. As a homotetrameric manganese enzyme, SOD2 plays a unique and indispensable role in protecting mitochondrial function, and SOD2 deficiency or genetic mutations can result in severe conditions such as dilated cardiomyopathy and mitochondrial dysfunction. Anti-SOD2 antibody (E-7) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), offering researchers a reliable tool for studying SOD2′s critical functions and regulation. Anti-SOD2 antibody (E-7) is available in both non-conjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing flexibility for diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Superoxide Dismutase 2/SOD2 Antibody (E-7) References:

1. Photoactivated hypericin increases the expression of SOD-2 and makes MCF-7 cells resistant to photodynamic therapy.  |  Kimáková, P., et al. 2017. Biomed Pharmacother. 85: 749-755. PMID: 27923686
2. Association between SOD2 V16A variant and urological cancer risk.  |  Zhang, LF., et al. 2020. Aging (Albany NY). 12: 825-843. PMID: 31929112
3. Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and Tissues.  |  Rath-Deschner, B., et al. 2021. Int J Mol Sci. 22: PMID: 33435582
4. Val16A SOD2 Polymorphism Promotes Epithelial-Mesenchymal Transition Antagonized by Muscadine Grape Skin Extract in Prostate Cancer Cells.  |  Sweeney, JD., et al. 2021. Antioxidants (Basel). 10: PMID: 33535682
5. Valproic acid promotes SOD2 acetylation: a potential mechanism of valproic acid-induced oxidative stress in developing systems.  |  Hansen, JM., et al. 2021. Free Radic Res. 55: 1130-1144. PMID: 34895005
6. Type-2 diabetic rat heart: The effect of kolaviron on mTOR-1, P70S60K, PKC-α, NF-kB, SOD-2, NRF-2, eNOS, AKT-1, ACE, and P38 MAPK gene expression profile.  |  Adoga, JO., et al. 2022. Biomed Pharmacother. 148: 112736. PMID: 35202911
7. Transcription factor Nrf2 binds to circRNAPIBF1 to regulate SOD2 in lung adenocarcinoma progression.  |  Zhang, Z., et al. 2022. Mol Carcinog. 61: 1161-1176. PMID: 36193777
8. Site 1 protease aggravates acute kidney injury by promoting tubular epithelial cell ferroptosis through SIRT3-SOD2-mtROS signaling.  |  Xie, S., et al. 2024. FEBS J. 291: 1575-1592. PMID: 38243371
9. Nesfatin-1 mitigates calcific aortic valve disease via suppressing ferroptosis mediated by GSH/GPX4 and ZIP8/SOD2 axes.  |  Wang, S., et al. 2024. Free Radic Biol Med. 222: 149-164. PMID: 38851518
10. Tissue-specific developmental regulation of superoxide dismutase (SOD-1 and SOD-2) activities in genetic strains of mice.  |  Schisler, NJ. and Singh, SM. 1985. Biochem Genet. 23: 291-308. PMID: 4015619

The preferred Superoxide Dismutase 2/SOD2 antibody is Superoxide Dismutase 2/SOD2 Antibody (A-2): sc-133134.