SLC35A5 Double Nickase Plasmid (h): sc-409546-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC35A5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC35A5 Double Nickase Plasmid (h) and SLC35A5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC35A5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC35A5 Double Nickase Plasmid (h) | sc-409546-NIC | 20 µg | $410.00 | |||
SLC35A5 Double Nickase Plasmid (h2) | sc-409546-NIC-2 | 20 µg | $410.00 |
SLC35A5 encodes a predicted nucleotide-sugar transporter of the solute carrier family that localizes to the secretory pathway and supports glycosylation by supplying activated sugar donors to luminal glycosyltransferases. By influencing the availability of nucleotide-sugars in the endoplasmic reticulum and Golgi, SLC35A5 can modulate glycan assembly on membrane and secreted proteins, with downstream effects on protein folding, trafficking, and cell–cell interactions. Altered glycosylation is a common feature of developmental and neurological disorders as well as cancer-associated phenotypes, making SLC35A5 relevant for studying how nucleotide-sugar transport shapes cellular homeostasis. Research applications include dissecting organelle-specific glycosylation pathways, mapping glycoproteomic changes, and evaluating impacts on secretion and receptor maturation in human cell models.
SLC35A5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC35A5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC35A5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC35A5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC35A5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.