Skip Antibody (D-5): sc-393856

Skip Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Skip protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Skip (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Skip protein, also known as Ski-interacting protein, plays a crucial role in cellular processes by acting as a nuclear hormone receptor that binds to the highly conserved region of Ski, which is essential for transforming activity. This interaction facilitates Vitamin D-mediated transcription, linking Skip protein to vital regulatory pathways in cellular differentiation and transformation. Notably, Skip protein has been shown to interact with E7, the major transforming protein of human papillomavirus, which is a significant factor in the development of cervical cancer. Furthermore, Skip protein exhibits specific inhibitory effects on bone morphogenetic protein-2-induced differentiation and is recognized as a novel regulator of differentiation programming induced by transforming growth factor-beta signals. Additionally, Skip protein functions as a repressor in Notch signaling, working in conjunction with the corepressor SMRT, thereby underscoring Skip′s multifaceted role in cellular signaling and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Skip Antibody (D-5) References:

1. The product of an oculopharyngeal muscular dystrophy gene, poly(A)-binding protein 2, interacts with SKIP and stimulates muscle-specific gene expression.  |  Kim, YJ., et al. 2001. Hum Mol Genet. 10: 1129-39. PMID: 11371506
2. Skip residues and charge interactions in myosin II coiled-coils: implications for molecular packing.  |  Straussman, R., et al. 2005. J Mol Biol. 353: 613-28. PMID: 16181641
3. Silencer of death domains (SODD) inhibits skeletal muscle and kidney enriched inositol 5-phosphatase (SKIP) and regulates phosphoinositide 3-kinase (PI3K)/Akt signaling to the actin cytoskeleton.  |  Rahman, P., et al. 2011. J Biol Chem. 286: 29758-70. PMID: 21712384
4. Regulation of insulin signaling and glucose transporter 4 (GLUT4) exocytosis by phosphatidylinositol 3,4,5-trisphosphate (PIP3) phosphatase, skeletal muscle, and kidney enriched inositol polyphosphate phosphatase (SKIP).  |  Ijuin, T. and Takenawa, T. 2012. J Biol Chem. 287: 6991-9. PMID: 22247557
5. Skip residues correlate with bends in the myosin tail.  |  Offer, G. 1990. J Mol Biol. 216: 213-8. PMID: 2254921
6. Characterization of porcine SKIP gene in skeletal muscle development: polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells.  |  Xiong, Q., et al. 2012. Meat Sci. 92: 490-7. PMID: 22682687
7. Role of phosphatidylinositol 3,4,5-trisphosphate (PIP3) 5-phosphatase skeletal muscle- and kidney-enriched inositol polyphosphate phosphatase (SKIP) in myoblast differentiation.  |  Ijuin, T. and Takenawa, T. 2012. J Biol Chem. 287: 31330-41. PMID: 22815484
8. Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78.  |  Ijuin, T., et al. 2015. Biochim Biophys Acta. 1853: 3192-201. PMID: 26376412
9. Phosphatidylinositol 3,4,5-Trisphosphate Phosphatase SKIP Links Endoplasmic Reticulum Stress in Skeletal Muscle to Insulin Resistance.  |  Ijuin, T., et al. 2016. Mol Cell Biol. 36: 108-18. PMID: 26483413
10. Glucose-regulated protein 78 (GRP78) binds directly to PIP3 phosphatase SKIP and determines its localization.  |  Ijuin, T., et al. 2016. Genes Cells. 21: 457-65. PMID: 26940976

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Skip Antibody (D-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Skip Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Skip Antibody (D-5) Specifications

Alternate Product Name: Skip

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 237-536

Epitope Target: Skip

Epitope Species: human

Product Reactivity/Detection: Skip

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Skip Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin