Six5 CRISPR/Cas9 KO Plasmid (h): sc-410513
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Six5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Six5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Six5 CRISPR/Cas9 KO Plasmid (h) | sc-410513 | 20 µg | $397.00 |
Overview
SIX5 encodes the human Six5 homeobox transcription factor, a DNA-binding regulator that helps coordinate gene expression programs during development and tissue differentiation. Six5 is associated with control of transcriptional networks governing cell fate decisions and can interface with pathways linked to cell-cycle progression and stress-responsive gene regulation. Altered SIX5 dosage or dysregulated expression has been implicated in disease-relevant phenotypes, including features associated with myotonic dystrophy type 1 through effects on neighboring loci and shared regulatory landscapes. Because Six5 influences lineage-specific transcription and cellular homeostasis, it is frequently studied in models of developmental biology, neuromuscular function, and transcriptional dysregulation.
Six5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SIX5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SIX5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SIX5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Six5 protein expression.
This CRISPR knockout system enables efficient generation of SIX5-deficient cell models for investigation of Six5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SIX5 exon(s) critical for Six5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SIX5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Six5 CRISPR/Cas9 KO Plasmid (h) and Six5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SIX5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Six5 HDR Plasmid (h) and Six5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SIX5 homology arms to support homology-directed repair at defined SIX5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.