SIT Antibody (F-9): sc-271933
- SIT Antibody (F-9) is a mouse monoclonal IgG2b κ SIT antibody provided at 200 µg/ml
- raised against amino acids 1-196 representing full length SIT of human origin
- SIT Antibody (F-9) is recommended for detection of SIT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SIT Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SIT Antibody (F-9) for WB applications. These reagents are now offered in bundles with SIT Antibody (F-9) (see ordering information below).
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SEE ALSO...
SIT Antibody (F-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects SIT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SIT (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIT protein, known as SHP2 interacting transmembrane adaptor protein, plays a crucial role in T lymphocyte signaling by acting as a disulfide-linked homodimeric glycoprotein that facilitates the recruitment of SH2 domain-containing intracellular signaling molecules to the plasma membrane through tyrosine-based signaling pathways. SIT presence in lymphocytes is vital for effective immune responses, as SIT contains five potential tyrosine phosphorylation sites that indicate involvement in T cell receptor-mediated signaling. Additionally, SIT interacts with key proteins such as SHP2 and the adaptor protein Grb2, and serves as a substrate for Src family kinases, including Fyn, Lck, and ZAP-70, highlighting SIT importance in modulating T cell activation and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SIT Antibody (F-9) References:
- SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. | Marie-Cardine, A., et al. 1999. J Exp Med. 189: 1181-94. PMID: 10209036
- The role of membrane-associated adaptors in T cell receptor signalling. | Zhang, W. and Samelson, LE. 2000. Semin Immunol. 12: 35-41. PMID: 10723796
- Antigen specific T lymphocyte activation. | Judd, BA. and Koretzky, GA. 2000. Rev Immunogenet. 2: 164-74. PMID: 11258415
- Structural and functional dissection of the cytoplasmic domain of the transmembrane adaptor protein SIT (SHP2-interacting transmembrane adaptor protein). | Pfrepper, KI., et al. 2001. Eur J Immunol. 31: 1825-36. PMID: 11433379
- Allergen-related approaches to immunotherapy. | Rolland, JM., et al. 2009. Pharmacol Ther. 121: 273-84. PMID: 19111571
- Novel immunotherapeutic approaches for allergy and asthma. | O'Mahony, L., et al. 2010. Autoimmunity. 43: 493-503. PMID: 20380589
- Allergen-specific immunotherapy: from therapeutic vaccines to prophylactic approaches. | Valenta, R., et al. 2012. J Intern Med. 272: 144-57. PMID: 22640224
- An ELISA for antisperm antibody detection in serum: comparison with TAT and SIT in serum, with MAR-test, immunobead-test and TAT in semen and with micro-SIT in cervical mucus. | Cimino, C., et al. 1987. Acta Eur Fertil. 18: 11-9. PMID: 3630564
- The molecular basis of the neutralization breadth of the RBD-specific antibody CoV11. | Tolbert, WD., et al. 2023. Front Immunol. 14: 1178355. PMID: 37334379
- Study of stereotyped, allergen-specific IgE sequences from specific immunotherapy participants yields new insight into mechanisms of allergy suppression. | Hwangpo, TA. and Schroeder, HW. 2023. J Allergy Clin Immunol. 152: 604-606. PMID: 37343844
- Concurrent immune checkpoint inhibition and selective immunosuppressive therapy in patients with immune-related enterocolitis. | Badran, YR., et al. 2023. J Immunother Cancer. 11: PMID: 37349130
- Mosunetuzumab in combination with CHOP in previously untreated DLBCL: safety and efficacy results from a phase 2 study. | Olszewski, AJ., et al. 2023. Blood Adv. 7: 6055-6065. PMID: 37581593
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIT Antibody (F-9) | sc-271933 | 200 µg/ml | $322.00 | |||
SIT Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-529290 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIT Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-522105 | 200 µg Ab, 40 µg BP | $361.00 | |||
SIT Antibody (F-9) AC | sc-271933 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SIT Antibody (F-9) HRP | sc-271933 HRP | 200 µg/ml | $322.00 | |||
SIT Antibody (F-9) FITC | sc-271933 FITC | 200 µg/ml | $336.00 | |||
SIT Antibody (F-9) PE | sc-271933 PE | 200 µg/ml | $349.00 | |||
SIT Antibody (F-9) Alexa Fluor® 488 | sc-271933 AF488 | 200 µg/ml | $364.00 | |||
SIT Antibody (F-9) Alexa Fluor® 546 | sc-271933 AF546 | 200 µg/ml | $364.00 | |||
SIT Antibody (F-9) Alexa Fluor® 594 | sc-271933 AF594 | 200 µg/ml | $364.00 | |||
SIT Antibody (F-9) Alexa Fluor® 647 | sc-271933 AF647 | 200 µg/ml | $364.00 | |||
SIT Antibody (F-9) Alexa Fluor® 680 | sc-271933 AF680 | 200 µg/ml | $364.00 | |||
SIT Antibody (F-9) Alexa Fluor® 790 | sc-271933 AF790 | 200 µg/ml | $364.00 |