Shank2 CRISPR/Cas9 KO Plasmid (m): sc-431686
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Shank2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Shank2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shank2 CRISPR/Cas9 KO Plasmid (m) | sc-431686 | 20 µg | $397.00 |
Overview
Shank2 encodes SH3 and multiple ankyrin repeat domains protein 2 (SHANK2), a postsynaptic density scaffold that organizes glutamatergic synapses by linking ionotropic receptors and adhesion complexes to downstream signaling and the actin cytoskeleton. In neurons, SHANK2 helps coordinate synaptic maturation, spine morphogenesis, and activity-dependent remodeling through interactions with PSD-95 family proteins, Homer–mGluR complexes, and actin-regulatory pathways. Disruption of Shank2-dependent scaffolding can alter excitatory synaptic transmission and synapse homeostasis, making it a frequently studied node in neuronal connectivity and plasticity programs. Genetic and functional studies have associated SHANK2 perturbation with neurodevelopmental phenotypes, supporting its relevance for modeling synaptic dysfunction mechanisms in vivo and in cultured mouse neurons.
Shank2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Shank2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Shank2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Shank2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Shank2 protein expression.
This CRISPR knockout system enables efficient generation of Shank2-deficient cell models for investigation of Shank2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Shank2 exon(s) critical for Shank2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Shank2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Shank2 CRISPR/Cas9 KO Plasmid (m) and Shank2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Shank2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Shank2 HDR Plasmid (m) and Shank2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Shank2 homology arms to support homology-directed repair at defined Shank2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.