Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SerpinA6 CRISPR/Cas9 KO Plasmid (h): sc-407031
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SerpinA6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SerpinA6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SerpinA6 Antibody (Y19-E): sc-100851
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SerpinA6 CRISPR/Cas9 KO Plasmid (h) | sc-407031 | 20 µg | $397.00 |
Overview
SERPINA6 encodes SerpinA6, also known as corticosteroid-binding globulin, a secreted serine protease inhibitor family member that primarily regulates systemic glucocorticoid bioavailability by binding and transporting cortisol in plasma. By buffering free cortisol levels, SerpinA6 influences glucocorticoid receptor signaling and downstream transcriptional programs that shape metabolism, immune responses, and stress physiology. Altered SERPINA6 expression or genetic variation has been associated with dysregulated hypothalamic–pituitary–adrenal axis activity and cortisol-related phenotypes, including susceptibility to metabolic and inflammatory traits. In experimental systems, SERPINA6 provides a handle to dissect endocrine signaling crosstalk with hepatic secretory pathways and extracellular protein homeostasis.
SerpinA6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINA6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINA6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINA6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SerpinA6 protein expression.
This CRISPR knockout system enables efficient generation of SERPINA6-deficient cell models for investigation of SerpinA6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINA6 exon(s) critical for SerpinA6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINA6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SerpinA6 CRISPR/Cas9 KO Plasmid (h) and SerpinA6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINA6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SerpinA6 HDR Plasmid (h) and SerpinA6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINA6 homology arms to support homology-directed repair at defined SERPINA6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.