RNF123 Double Nickase Plasmid (h): sc-403631-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF123 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF123 Double Nickase Plasmid (h) and RNF123 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF123. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RNF123 Antibody (267.1): sc-101122
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF123 Double Nickase Plasmid (h) | sc-403631-NIC | 20 µg | $410.00 | |||
RNF123 Double Nickase Plasmid (h2) | sc-403631-NIC-2 | 20 µg | $410.00 |
RNF123 encodes an E3 ubiquitin-protein ligase that regulates protein turnover through ubiquitin-dependent proteasomal degradation and coordinates substrate recognition within ubiquitin signaling networks. By controlling the stability of select regulatory proteins, RNF123 influences cell cycle progression, cellular stress responses, and pathways linked to proteostasis and signal transduction. Perturbation of ubiquitin ligase activity can remodel transcriptional programs and checkpoint control, making RNF123 a relevant node for mechanistic studies of dysregulated growth and genome surveillance. Altered RNF123 expression or function has been reported in contexts of human disease biology where ubiquitination-mediated control of protein homeostasis is disrupted.
RNF123 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF123 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF123. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF123 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF123-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.