Ribosomal Protein LP0 CRISPR/Cas9 KO Plasmid (m): sc-419183

Overview

Rplp0 encodes ribosomal protein LP0 (P0), a core component of the 60S large ribosomal subunit that forms the ribosomal stalk with P1/P2 proteins and supports translation factor recruitment and GTPase activation during elongation. As a conserved housekeeping ribosomal protein, LP0 is essential for ribosome biogenesis, global protein synthesis, and cellular growth and stress adaptation programs linked to nucleolar function. Perturbation of ribosomal proteins can disrupt translational control and proteostasis, contributing to ribosomopathy-like phenotypes and altered proliferation, apoptosis, and differentiation in disease-relevant contexts. In mouse systems, Rplp0 is also widely used as a reference gene, making targeted disruption informative for evaluating normalization strategies and translation-dependent phenotypes.

Ribosomal Protein LP0 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rplp0 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rplp0 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rplp0 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein LP0 protein expression.

This CRISPR knockout system enables efficient generation of Rplp0-deficient cell models for investigation of Ribosomal Protein LP0 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Rplp0 exon(s) critical for Ribosomal Protein LP0 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Rplp0 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Ribosomal Protein LP0 CRISPR/Cas9 KO Plasmid (m) and Ribosomal Protein LP0 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rplp0 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Ribosomal Protein LP0 HDR Plasmid (m) and Ribosomal Protein LP0 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rplp0 homology arms to support homology-directed repair at defined Rplp0 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.