Anticorps rhodopsin (1D4) recommandé pour la détection de rhodopsin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps rhodopsin (1D4): sc-57432

Anticorps rhodopsin (1D4) est évalué 4.4 de 5 de 11.
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Fiches techniques

    • Anti-L'anticorps rhodopsin 1D4 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps rhodopsin fourni en 200 µg/ml
    • dirigé contre la protéine totale rhodopsin de bovine
    • Anti-L'Anticorps rhodopsin (1D4) est recommandé pour la détection de rhodopsin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont bovine
    • Anti-L'Anticorps rhodopsin (1D4) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de rhodopsin (1D4): sc-57432.
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Would this antibody recognize the 1D4 tag in fusion protein in western blot?

Posée par: rogomca
Thank you for your question. This is a mouse monoclonal antibody that is recommended for detection of the protein rhodopsin. The clone name for this antibody is “1D4”, which is also known as the “1D4” epitope of rhodopsin. We have not tested this antibody for detection of any 1D4 fusion proteins.
Répondue par: Technical Support
Date de publication: 2019-08-17

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Posée par: Anonyme
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody rhodopsin (1D4): sc-57432 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Répondue par: Technical Support 10
Date de publication: 2017-01-09
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Rated 4 de 5 de par Visualizing Endogenous Rhodopsin in RPE Cells Useful antibody for IF labeling of endogenous rhodopsin in RPE cells. Cells permeabilized for 30min in PBStween then blocked in 5%BSA. Antibody used at 1:200 overnight at 4 degrees.
Date de publication: 2020-02-14
Rated 4 de 5 de par Antibody for IF I used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 568 anti-mouse) at a concentration of 1:1000. Staining was fairly robust but not completely consistent between all samples.
Date de publication: 2018-03-21
Rated 2 de 5 de par Poor specificity in normal canine WB Antibody was used for WB with normal canine tissue. Very low specificity.
Date de publication: 2017-08-18
Rated 5 de 5 de par Great reactivity. This antibody will give you strong and specific signals in your blotting and staining. It even cross-reacts with additional species. It has been published with over 15 times.
Date de publication: 2017-01-11
Rated 5 de 5 de par Outstanding!! Great product for both western blot and ihc cell staining applications
Date de publication: 2017-01-05
Rated 4 de 5 de par Decent I bought this antibody for WB of rhodopsin in Y79 cells. So far it works as advertised.
Date de publication: 2016-12-12
Rated 4 de 5 de par Good specificity and signal strength with Good specificity and signal strength with some background in mouse eye and rat eye tissue extracts. -SCBT QC
Date de publication: 2015-06-01
Rated 5 de 5 de par This antibody was used in a publication for This antibody was used in a publication for WB on human HEK 293 and ARPE-19 cells - SCBT Publication Review
Date de publication: 2015-04-04
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