REPS1 CRISPR/Cas9 KO Plasmid (m): sc-422650
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- REPS1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the REPS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REPS1 CRISPR/Cas9 KO Plasmid (m) | sc-422650 | 20 µg | $397.00 |
Overview
Reps1 (REPS1) encodes an endocytic adaptor implicated in receptor internalization and membrane trafficking through interactions with proteins involved in clathrin-mediated endocytosis and small GTPase signaling. REPS1 participates in spatial control of signaling by regulating recycling and downregulation of activated cell-surface receptors, influencing pathways that shape proliferation, adhesion, and cytoskeletal dynamics. In mouse systems, perturbation of Reps1 is used to interrogate how endocytic trafficking interfaces with growth factor and integrin-dependent signaling networks in immune and epithelial contexts. Dysregulated receptor trafficking and signal attenuation are broadly relevant to disease-associated mechanisms, including altered cell migration and aberrant signal transduction, making Reps1 a useful node for mechanistic studies.
REPS1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Reps1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Reps1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Reps1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish REPS1 protein expression.
This CRISPR knockout system enables efficient generation of Reps1-deficient cell models for investigation of REPS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Reps1 exon(s) critical for REPS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Reps1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by REPS1 CRISPR/Cas9 KO Plasmid (m) and REPS1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Reps1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by REPS1 HDR Plasmid (m) and REPS1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Reps1 homology arms to support homology-directed repair at defined Reps1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.