Date published: 2025-9-9

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Anticorps RAP55 (G-5): sc-398633

5.0(1)
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Fiches techniques
  • L'Anticorps RAP55 (G-5) est un monoclonal de souris IgG2b (chaîne légère kappa) fourni en 200 µg/ml
  • dirigé contre les acides aminés 103-197 dans une région interne de RAP55 d'origine human
  • recommandé pour la détection de RAP55 d'origine mouse, rat et human par WB, IP, IF et ELISA
  • m-IgG Fc BP-HRP, 2b BP-HRP">m-IgG2b BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps RAP55 (G-5) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps RAP55 (G-5)(voir les informations de commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    RAP55, also known as LSM14A (LSM14 homolog A), is a 463 amino acid member of the LSM14 family that contains a FFD-TFG box domain. The LSM14 domain and the RGG repeats of RAP55 are required for accumulation in P-bodies, and the region containing the FDF motif is responsible for cytoplasmic retention. RAP55, and all other Sm-like proteins, contain the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. RAP55 is phosphorylated upon DNA damage, probably by ATM or ATR. Existing as two alternatively spliced isoforms, the RAP55 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, mosquito, Drosophila, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea and N.crassa, and maps to human chromosome 19q13.11.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps RAP55 (G-5)

    sc-398633
    200 µg/ml
    $316.00

    RAP55 (G-5): m-IgG Fc BP-HRP Kit

    sc-538483
    200 µg Ab; 10 µg BP
    $354.00

    RAP55 (G-5): m-IgGκ BP-HRP Kit

    sc-536107
    200 µg Ab; 40 µg BP
    $354.00

    RAP55 (G-5): m-IgG2b BP-HRP Kit

    sc-549952
    200 µg Ab; 10 µg BP
    $354.00

    How much sample do you recommend loading on the gel for Western blot detection using RAP55 (G-5): sc-398633?

    Posée par: jerojero
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Répondue par: Technical Support
    Date de publication: 2017-03-01
    • y_2025, m_9, d_8, h_8CST
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    Rated 5 de 5 de par Nice Western blot data of RAP55 expression in nonNice Western blot data of RAP55 expression in non-transfected 293T, mouse RAP55 transfected 293T and HeLa whole cell lysates. -SCBT QC
    Date de publication: 2014-02-18
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    Anticorps RAP55 (G-5) est évalué 5.0 de 5 de 1.
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