PRX IV CRISPR/Cas9 KO Plasmid (m): sc-424704
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRX IV CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRX IV genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRX IV Antibody (F-2): sc-376668
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRX IV CRISPR/Cas9 KO Plasmid (m) | sc-424704 | 20 µg | $397.00 |
Overview
Prdx4 encodes peroxiredoxin IV (PRX IV), an endoplasmic reticulum–localized thiol-dependent peroxidase that reduces hydrogen peroxide and organic hydroperoxides to maintain redox homeostasis in secretory and membrane protein–producing cells. In the ER lumen, PRX IV helps shape oxidative protein folding by coupling peroxide detoxification to disulfide bond formation, influencing proteostasis and unfolded protein response signaling during ER stress. Through control of local reactive oxygen species, PRX IV can modulate redox-sensitive pathways that affect inflammation, cell survival, and metabolic adaptation. Dysregulated ER redox balance and PRX IV activity have been associated with oxidative stress–linked phenotypes relevant to cancer biology, metabolic disease, and inflammatory processes, making Prdx4 a useful node for mechanistic studies in mouse systems.
PRX IV CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prdx4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prdx4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prdx4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRX IV protein expression.
This CRISPR knockout system enables efficient generation of Prdx4-deficient cell models for investigation of PRX IV signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prdx4 exon(s) critical for PRX IV function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prdx4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRX IV CRISPR/Cas9 KO Plasmid (m) and PRX IV CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prdx4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRX IV HDR Plasmid (m) and PRX IV HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prdx4 homology arms to support homology-directed repair at defined Prdx4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.