PRKCDBP CRISPR/Cas9 KO Plasmid (h): sc-407370
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRKCDBP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRKCDBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRKCDBP Antibody (8D3): sc-293329
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRKCDBP CRISPR/Cas9 KO Plasmid (h) | sc-407370 | 20 µg | $397.00 |
Overview
PRKCDBP (protein kinase C delta binding protein) is a cytosolic adaptor implicated in PKCδ-associated signaling, where it influences phosphorylation-dependent pathways that regulate cell-cycle progression, apoptosis, and stress responses. The protein has been linked to modulation of transcriptional programs and checkpoint control, integrating upstream cues from kinase cascades that shape cellular growth and survival decisions. Altered PRKCDBP expression has been reported across multiple tumor contexts, supporting its study in mechanisms of proliferation control, DNA damage responses, and tumor suppressor–associated networks. These properties make PRKCDBP a relevant target for dissecting signaling dependencies and context-specific phenotypes in human cell models.
PRKCDBP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRKCDBP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRKCDBP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRKCDBP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRKCDBP protein expression.
This CRISPR knockout system enables efficient generation of PRKCDBP-deficient cell models for investigation of PRKCDBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRKCDBP exon(s) critical for PRKCDBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRKCDBP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRKCDBP CRISPR/Cas9 KO Plasmid (h) and PRKCDBP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRKCDBP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRKCDBP HDR Plasmid (h) and PRKCDBP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRKCDBP homology arms to support homology-directed repair at defined PRKCDBP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.