PRK2 CRISPR/Cas9 KO Plasmid (m): sc-430934
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRK2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRK2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRK2 Antibody (A-3): sc-271971
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRK2 CRISPR/Cas9 KO Plasmid (m) | sc-430934 | 20 µg | $397.00 |
Overview
Mouse Pkn2 encodes protein kinase C-related kinase 2 (PRK2), a serine/threonine kinase in the AGC family that functions as a Rho GTPase effector linking cytoskeletal remodeling to signal transduction. PRK2 participates in pathways regulating actin dynamics, focal adhesion turnover, cell polarity, and stress-responsive signaling, with additional roles reported in transcriptional control and cell-cycle progression. Through interactions with RhoA and associated scaffold proteins, PRK2 can influence migration and morphogenesis programs that are frequently interrogated in developmental and cancer biology. Dysregulated PRK2-associated signaling has been connected to altered proliferative and invasive phenotypes in model systems, supporting its relevance for mechanistic studies of disease-related cellular states.
PRK2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pkn2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pkn2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pkn2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRK2 protein expression.
This CRISPR knockout system enables efficient generation of Pkn2-deficient cell models for investigation of PRK2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pkn2 exon(s) critical for PRK2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pkn2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRK2 CRISPR/Cas9 KO Plasmid (m) and PRK2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pkn2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRK2 HDR Plasmid (m) and PRK2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pkn2 homology arms to support homology-directed repair at defined Pkn2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.