Progesterone Receptor Antibody (2C11F11): sc-130071

PR Antibody (2C11F11) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect both PR-A and PR-B isoforms of the human progesterone receptor across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-progesterone receptor antibody (2C11F11) is raised against a recombinant protein corresponding to amino acids 731-909 of the human progesterone receptor, targeting a region crucial for receptor structure and function. Progesterone receptor (2C11F11) monoclonal antibody recognizes a nuclear hormone receptor with a modular structure, including DNA-binding and ligand-binding domains essential for gene transcription regulation in response to progesterone binding. PR-A and PR-B isoforms are transcribed from separate, estrogen-inducible promoters within a single PR gene. PR-B contains an additional 164 amino acids at its N-terminus compared to PR-A, resulting in different transcriptional activation properties. PR-B functions as a potent transcriptional activator across various cellular and promoter contexts, while PR-A acts as a ligand-dependent transdominant repressor, modulating other steroid hormone receptors′ activity. An inhibitory domain present in both isoforms is only active in PR-A, highlighting their distinct cellular conformations. Progesterone receptor monoclonal antibody (2C11F11) serves as a reliable tool for investigating these mechanisms in detail.

The preferred Progesterone Receptor antibody is Progesterone Receptor Antibody (AB-52): sc-810.