Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
podoplanin Double Nickase Plasmid (h): sc-401332-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- podoplanin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- podoplanin Double Nickase Plasmid (h) and podoplanin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDPN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: podoplanin Antibody (E-1): sc-376695
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
podoplanin Double Nickase Plasmid (h) | sc-401332-NIC | 20 µg | $410.00 | |||
podoplanin Double Nickase Plasmid (h2) | sc-401332-NIC-2 | 20 µg | $410.00 |
Human PDPN encodes podoplanin, a mucin-type transmembrane glycoprotein that modulates cell shape, adhesion, and motility through interactions with ERM proteins and remodeling of the actin cytoskeleton. Podoplanin also functions as a key ligand for CLEC-2, linking PDPN-expressing cells to platelet activation and contributing to lymphatic and vascular-associated signaling processes. PDPN expression is enriched in lymphatic endothelium and stromal compartments, where it participates in tissue organization, epithelial–mesenchymal-like programs, and extracellular matrix dynamics. Dysregulated podoplanin has been associated with tumor-associated stroma, invasive phenotypes, and inflammatory remodeling, making PDPN a useful target for mechanistic studies of microenvironmental signaling.
podoplanin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDPN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDPN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDPN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDPN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.