Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLC β1 CRISPR/Cas9 KO Plasmid (h): sc-401141
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLC β1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLC β1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLC β1 Antibody (D-8): sc-5291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLC β1 CRISPR/Cas9 KO Plasmid (h) | sc-401141 | 20 µg | $397.00 |
Overview
PLCB1 encodes phospholipase C beta 1 (PLCβ1), a G protein–regulated effector enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate inositol 1,4,5-trisphosphate and diacylglycerol, thereby mobilizing intracellular Ca2+ and activating protein kinase C signaling. This pathway integrates inputs from GPCRs to regulate diverse processes including neuronal excitability, synaptic signaling, secretion, and cytoskeletal dynamics. PLCβ1 activity contributes to downstream MAPK and calcium-dependent transcriptional programs, linking membrane receptor signaling to changes in gene expression and cellular state. Altered PLCB1 expression or signaling has been associated with neuropsychiatric phenotypes and aberrant growth-control signaling, supporting its use in mechanistic studies of signal transduction and disease-relevant cellular models.
PLC β1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLCB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLCB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLCB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLC β1 protein expression.
This CRISPR knockout system enables efficient generation of PLCB1-deficient cell models for investigation of PLC β1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLCB1 exon(s) critical for PLC β1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLCB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLC β1 CRISPR/Cas9 KO Plasmid (h) and PLC β1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLCB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLC β1 HDR Plasmid (h) and PLC β1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLCB1 homology arms to support homology-directed repair at defined PLCB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.