Pim-1 Double Nickase Plasmid (m): sc-422237-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pim-1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Pim-1 Double Nickase Plasmid (m) and Pim-1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pim1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pim-1 Antibody (G-11): sc-374116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pim-1 Double Nickase Plasmid (m) | sc-422237-NIC | 20 µg | $410.00 | |||
Pim-1 Double Nickase Plasmid (m2) | sc-422237-NIC-2 | 20 µg | $410.00 |
Mouse Pim1 encodes the Pim-1 serine/threonine kinase, a constitutively active signaling enzyme that modulates cell survival, proliferation, and metabolic adaptation. Pim-1 phosphorylates substrates involved in cell-cycle control and apoptosis, including regulators of translation and stress responses, and it cooperates with growth factor and cytokine signaling to shape hematopoietic and immune cell function. As a downstream effector frequently linked to oncogenic transcriptional programs, Pim-1 is studied in pathways that converge on MYC activity, mTOR-dependent protein synthesis, and resistance to cellular stress. Dysregulated Pim1/Pim-1 activity has been associated with malignant transformation and tumor progression in multiple model systems, making it a common target for mechanistic studies of kinase signaling and oncogenesis.
Pim-1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pim1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pim1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pim1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pim1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.