Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PGP Antibody (E-10) is a high quality monoclonal PGP antibody (also designated PGP antibody) suitable for the detection of the PGP protein of mouse, rat and human origin. PGP Antibody (E-10) is available as both the non-conjugated anti-PGP antibody form, as well as multiple conjugated forms of anti-PGP antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. PGP (phosphoglycolate phosphatase), also known as PGPase, is a 321 amino acid enzyme belonging to the HAD-like hydrolase superfamily and the CbbY/cbbZ/gph/yieH family. PGP is detected in all tissues including red cells, lymphocytes and cultured fibroblasts, with highest activity found in skeletal and cardiac muscle. PGP is considered an important regulatory enzyme on oxygen transport by indirectly affecting the level of red cell 2,3-diphosphoglycerate. The gene encoding PGP maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn′s disease, which is a gastrointestinal inflammatory condition.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I am using PGP (E-10): sc-390883 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
Posée par: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support 8
Date de publication: 2017-01-20
what is the second antibody we could use for this new PGP antibody(E-10)??
Posée par: muves
Thank you for your question. You can use any anti-mouse secondary antibody reagent. We offer a brand new product to use against this mouse monoclonal antibody:
These are mouse IgG binding proteins and are strongly recommended alternatives to conventional goat/rabbit anti-mouse IgG secondary antibodies. Because they are highly specific reagents, they provide strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies.
We would be happy to send you a free sample of any of these reagents! Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Répondue par: Tech Service
Date de publication: 2016-10-26
I have a question about sc-390883, I want to use this antibody for western blotting and I want to know what dilution I should use from this?
Posée par: melika
Thank you for your question. We recommend using this antibody, sc-390883: PGP (E-10), at a starting dilution of 1:200 in Western blotting, with a optimal range between 1:100-1:1,000, which should be determined through titration.
Rated 5 de
Medo Medo par
Great resultI bought the Pgp Ab this month and I got great result , so I'm highly recommend working with these company products.
Date de publication: 2017-05-27
Rated 5 de
ExcellentGreat product for both western blot and IHC studies
Date de publication: 2017-02-03
Rated 5 de
Excellent!This antibody works great in western blot analysis of PGP expression in human heart and human skeletal muscle tissue extracts and K-562 whole cell lysate.
Date de publication: 2017-01-12
Rated 5 de
Excellent antibody in WB (1:1000) on humanExcellent antibody in WB (1:1000) on human cell lines. Knockout of PGP leads to complete absence of signal, indicating excellent specificity.
Date de publication: 2016-09-20
Rated 5 de
Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date de publication: 2015-01-08
Rated 5 de
Nice Western Blot data of PGP expressionNice Western Blot data of PGP expression in human heart and human skeletal muscle tissue extracts and K-562 whole cell lysate . -SCBT QC
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