Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK3 Double Nickase Plasmid (h): sc-404502-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDK3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PDK3 Double Nickase Plasmid (h) and PDK3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDK3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDK3 Antibody (A-4): sc-365378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK3 Double Nickase Plasmid (h) | sc-404502-NIC | 20 µg | $410.00 | |||
PDK3 Double Nickase Plasmid (h2) | sc-404502-NIC-2 | 20 µg | $410.00 |
PDK3 encodes pyruvate dehydrogenase kinase isoform 3, a mitochondrial Ser/Thr kinase that phosphorylates and inhibits the pyruvate dehydrogenase complex, thereby limiting conversion of pyruvate to acetyl-CoA and shifting carbon flux away from the TCA cycle. Through this control point, PDK3 integrates nutrient availability and hypoxia signaling with glucose oxidation, lactate production, and broader mitochondrial bioenergetics. Dysregulated PDK3 activity has been linked to pathological metabolic reprogramming and altered redox balance, with reported associations to cancer cell metabolism and neuromuscular and peripheral nerve disorders. As a regulator of oxidative metabolism, PDK3 is frequently studied in contexts involving mitochondrial stress responses, glycolytic dependence, and metabolic adaptation.
PDK3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDK3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDK3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDK3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDK3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.