Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PC-PLD2 Double Nickase Plasmid (h): sc-402056-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PC-PLD2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PC-PLD2 Double Nickase Plasmid (h) and PC-PLD2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLD2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PC-PLD2 Antibody (B-3): sc-515744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PC-PLD2 Double Nickase Plasmid (h) | sc-402056-NIC | 20 µg | $410.00 | |||
PC-PLD2 Double Nickase Plasmid (h2) | sc-402056-NIC-2 | 20 µg | $410.00 |
Human PLD2 encodes phospholipase D2 (PC-PLD2), a membrane-associated enzyme that hydrolyzes phosphatidylcholine to generate phosphatidic acid, a lipid second messenger that shapes membrane curvature and signaling microdomains. PLD2 activity interfaces with receptor-driven pathways including EGFR, GPCR, and small GTPase signaling, influencing vesicle trafficking, endocytosis, actin cytoskeletal remodeling, and cell migration. Through phosphatidic acid–dependent regulation of mTOR and MAPK signaling, PLD2 contributes to metabolic and growth-related cellular responses and has been linked to inflammatory signaling outputs. Dysregulated PLD2 signaling has been reported across contexts such as tumor cell invasiveness, immune cell chemotaxis, and neuroinflammatory processes, making it a useful node for mechanistic studies of lipid-mediated signaling.
PC-PLD2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.