p41-ARCb CRISPR Activation Plasmid (m): sc-419207-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p41-ARCb CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- p41-ARCb CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by p41-ARCb CRISPR Activation Plasmid (m) and p41-ARCb CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Arpc1b transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p41-ARCb Antibody (C-3): sc-137125
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p41-ARCb CRISPR Activation Plasmid (m) | sc-419207-ACT | 20 µg | $397.00 |
Arpc1b encodes p41-ARCb, an ARP2/3 complex subunit that promotes actin nucleation and branching required for dynamic cytoskeletal remodeling in mouse cells. By supporting lamellipodia formation, endocytosis, and vesicular trafficking, p41-ARCb contributes to processes such as immune cell migration, phagocytosis, and adhesion-dependent signaling. ARP2/3-driven actin networks interface with Rho-family GTPase pathways and downstream regulators of cell polarity and motility. Dysregulation of ARPC1B-associated actin assembly is linked to altered immune function and inflammatory phenotypes, making it relevant for studying cytoskeletal contributions to host defense and tissue homeostasis.
p41-ARCb CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Arpc1b expression without altering the underlying DNA sequence.
p41-ARCb CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Arpc1b locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Arpc1b transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous p41-ARCb expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Arpc1b locus and enabling the study of p41-ARCb-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of p41-ARCb pathway restoration in tumor cells with silenced or reduced Arpc1b expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.