Anticorps p-Shc (15E11) recommandé pour la détection de Tyr 317 phosphorylated Shc d'origine mouse, rat, human et canine par WB, IP et IF

Anticorps p-Shc (15E11): sc-81518

Anticorps p-Shc (15E11) est évalué 4.0 de 5 de 2.
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Fiches techniques

    • L'anticorps p-Shc 15E11 est un monoclonal IgG1 de souris fourni en 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding tyrosine 317 of Shc of human origin
    • recommandé pour la détection de Tyr 317 phosphorylated Shc d'origine mouse, rat, human et canine par WB, IP et IF
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Shc Antibody (15E11). This work is in progress.
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I am using p-Shc (15E11): sc-81518 for immunofluorescence, what dilution of this antibody should I use?

Posée par: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 4 de 5 de par Positive signal in WB in HEK293 whole cell lysates Positive signal in WB in HEK293 whole cell lysates. -SCBT QC
Date de publication: 2015-01-09
Rated 4 de 5 de par Strong band in WB in mouse transfected 293T Strong band in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2013-07-08
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