Date published: 2025-9-8

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Anticorps p-PYK2 (14F6): sc-81512

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Fiches techniques
  • L'anticorps p-PYK2 14F6 est un monoclonal IgG1 de souris, cité dans 3 publications, fourni en 50 µg/0.5 ml
  • soulevée contre un phosphopeptide synthétique correspondant aux résidus d'acides aminés entourant la tyrosine 402 de PYK2 d'origine human.
  • recommandé pour la détection de Tyr 402 phosphorylated PYK2 d'origine mouse, rat et human par WB et IP; pas de réaction croisée avec les non-phosphorylated form of PYK2 or with unrelated phosphorylation sites
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire p-PYK2 (14F6). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

PYK2 (proline-rich tyrosine kinase 2), a putative member of the FAK family, exhibits 61% sequence identity with FAK within its kinase domain. Like FAK, PYK2 has been shown to be a cytoplasmic protein tyrosine kinase, which is a substrate for the intrinsic protein tyrosine kinase activity of pp60Src. PYK2 (also designated CAKb or RAFTK) is highly expressed in the central nervous system. PYK2 is rapidly phosphorylated on tyrosine residues in response to stimuli, which increases intracellular calcium levels and, in turn, activates members of the PKC family of kinases. Specifically, PYK2 is phosphorylated on Tyr 402 after stimulation with heregulin. This promotes the formation of a multiprotein complex that mediates the phosphorylation of p190 RhoGAP by Src. Activation of the PYK2 kinase leads to modulation of ion channel function and the activation of the MAPK signaling pathway. PYK2 also contains phosphorylation sites within the activation loop at Tyr 579 and Tyr 580 and on the potential Grb2-binding site at Tyr 881.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps p-PYK2 (14F6)

sc-81512
50 µg/0.5 ml
$316.00

I am using p-PYK2 (14F6): sc-81512 antibody for immunoprecipitation, how much antibody should I use?

Posée par: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Répondue par: Technical Support
Date de publication: 2023-09-24
  • y_2025, m_8, d_29, h_8CST
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Rated 3 de 5 de par Worked with transfected lysate by WB withWorked with transfected lysate by WB with some background -SCBT QC
Date de publication: 2014-04-17
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Anticorps p-PYK2 (14F6) est évalué 3.0 de 5 de 1.
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