OTUD5 Double Nickase Plasmid (h): sc-412550-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUD5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OTUD5 Double Nickase Plasmid (h) and OTUD5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OTUD5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD5 Double Nickase Plasmid (h) | sc-412550-NIC | 20 µg | $410.00 | |||
OTUD5 Double Nickase Plasmid (h2) | sc-412550-NIC-2 | 20 µg | $410.00 |
OTUD5 (also known as DUBA) encodes a deubiquitinating enzyme of the OTU family that cleaves ubiquitin chains to regulate protein stability and signaling amplitude. It participates in ubiquitin-dependent control of innate immune and inflammatory pathways, including modulation of interferon responses and NF-κB-associated signaling nodes. OTUD5 also contributes to DNA damage and replication stress responses through ubiquitin editing at chromatin-associated substrates, influencing genome maintenance programs. Dysregulated OTUD5 activity or expression has been linked in the literature to immune dysfunction and cancer-associated signaling rewiring, making it a useful target for mechanistic studies of ubiquitin circuitry.
OTUD5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OTUD5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OTUD5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OTUD5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OTUD5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.