OTUD2 CRISPR/Cas9 KO Plasmid (m): sc-432624
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUD2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OTUD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD2 CRISPR/Cas9 KO Plasmid (m) | sc-432624 | 20 µg | $397.00 |
Overview
Yod1 encodes OTUD2, an OTU family deubiquitinating enzyme that hydrolyzes ubiquitin chains to regulate protein stability and signaling amplitude. OTUD2 participates in ubiquitin-dependent control of proteostasis and stress-responsive signaling pathways, including inflammatory and innate immune circuits where ubiquitination tunes pathway activation and termination. By reshaping ubiquitin signals, OTUD2 can influence cell survival decisions, DNA damage responses, and transcriptional programs linked to cellular homeostasis. Dysregulated ubiquitin editing has been implicated across cancer biology and immune-mediated disease mechanisms, making Yod1/OTUD2 a useful node for pathway dissection in mouse models.
OTUD2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Yod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Yod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Yod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OTUD2 protein expression.
This CRISPR knockout system enables efficient generation of Yod1-deficient cell models for investigation of OTUD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Yod1 exon(s) critical for OTUD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Yod1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OTUD2 CRISPR/Cas9 KO Plasmid (m) and OTUD2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Yod1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OTUD2 HDR Plasmid (m) and OTUD2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Yod1 homology arms to support homology-directed repair at defined Yod1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.