Olfr45 Double Nickase Plasmid (m2): sc-422035-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Olfr45 Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Olfr45 Double Nickase Plasmid (m2) and Olfr45 Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Olfr45. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Olfr45 Double Nickase Plasmid (m2) | sc-422035-NIC-2 | 20 µg | $410.00 |
Mouse Olfr45 encodes an olfactory receptor in the G protein–coupled receptor superfamily that participates in odorant recognition and signal transduction in the olfactory sensory epithelium. Upon ligand engagement, OLFR45 is expected to couple to heterotrimeric G proteins, modulating adenylate cyclase activity, cAMP production, and downstream ion channel activation to drive neuronal depolarization and olfactory circuit input. Altered expression or signaling of olfactory receptors is relevant to studies of sensory dysfunction and broader GPCR regulatory mechanisms, including receptor trafficking, desensitization, and transcriptional control during neuronal differentiation. Gene editing of Olfr45 supports functional genomics experiments such as mapping odorant–receptor specificity, dissecting GPCR signaling nodes in sensory neurons, and generating mouse models to interrogate olfaction-linked phenotypes and neuroepithelial homeostasis.
Olfr45 Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Olfr45 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Olfr45. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Olfr45 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Olfr45-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.