Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OCTN1 CRISPR/Cas9 KO Plasmid (h): sc-403258
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OCTN1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OCTN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OCTN1 CRISPR/Cas9 KO Plasmid (h) | sc-403258 | 20 µg | $397.00 |
Overview
SLC22A4 encodes the human organic cation/carnitine transporter OCTN1 (SLC22 family), a multi-pass membrane protein that mediates uptake and efflux of zwitterions and organic cations, including carnitine-related substrates and the antioxidant ergothioneine. By regulating intracellular availability of these metabolites, OCTN1 contributes to cellular redox homeostasis, xenobiotic handling, and metabolic stress responses, with functional impact in epithelial, immune, and barrier-associated tissues. Genetic variation and altered expression of SLC22A4 have been linked to susceptibility and phenotypic modulation in inflammatory and immune-associated disorders, making it a relevant node for studying transport-driven signaling and environmental interactions.
OCTN1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC22A4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC22A4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC22A4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OCTN1 protein expression.
This CRISPR knockout system enables efficient generation of SLC22A4-deficient cell models for investigation of OCTN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC22A4 exon(s) critical for OCTN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC22A4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OCTN1 CRISPR/Cas9 KO Plasmid (h) and OCTN1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC22A4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OCTN1 HDR Plasmid (h) and OCTN1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC22A4 homology arms to support homology-directed repair at defined SLC22A4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.