Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NPC1L1 Double Nickase Plasmid (h): sc-401741-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPC1L1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NPC1L1 Double Nickase Plasmid (h) and NPC1L1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NPC1L1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NPC1L1 Antibody (G-1): sc-166802
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPC1L1 Double Nickase Plasmid (h) | sc-401741-NIC | 20 µg | $410.00 | |||
NPC1L1 Double Nickase Plasmid (h2) | sc-401741-NIC-2 | 20 µg | $410.00 |
NPC1L1 (Niemann-Pick C1-Like 1) is a multi-pass membrane protein that mediates cellular uptake of cholesterol and other sterols, coupling luminal lipid availability to intracellular trafficking and metabolic regulation. In human tissues, NPC1L1 supports sterol absorption and contributes to homeostatic control of cholesterol levels through coordinated interactions with endocytic transport routes and lipid-handling networks. Its activity is functionally linked to pathways governing sterol transport, membrane lipid composition, and lipoprotein metabolism. Dysregulation of NPC1L1-associated processes is relevant to studies of hypercholesterolemia, atherosclerosis risk biology, and metabolic disease mechanisms.
NPC1L1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NPC1L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NPC1L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NPC1L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NPC1L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.