NG2 Double Nickase Plasmid (m): sc-431202-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NG2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NG2 Double Nickase Plasmid (m) and NG2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cspg4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NG2 Antibody (LHM 2): sc-53389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NG2 Double Nickase Plasmid (m) | sc-431202-NIC | 20 µg | $410.00 | |||
NG2 Double Nickase Plasmid (m2) | sc-431202-NIC-2 | 20 µg | $410.00 |
Mouse Cspg4 encodes NG2 (also known as CSPG4), a transmembrane chondroitin sulfate proteoglycan enriched in oligodendrocyte progenitor cells and pericytes where it regulates cell adhesion, migration, and proliferation. NG2 interfaces with extracellular matrix components and integrin-linked signaling to modulate cytoskeletal dynamics and growth factor responsiveness, supporting vascular stabilization and white matter development. Dysregulated NG2/Cspg4 expression is frequently examined in contexts of glioma biology, demyelinating injury responses, and tumor-associated stromal remodeling, where it can influence cell state and tissue microenvironmental interactions. As a lineage and functional marker, NG2 is widely used to study progenitor maintenance, differentiation trajectories, and neurovascular unit organization in mouse models.
NG2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cspg4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cspg4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cspg4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cspg4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.